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Fig. 3 | BMC Pregnancy and Childbirth

Fig. 3

From: Development of a melting-curve based multiplex real-time PCR assay for simultaneous detection of Streptococcus agalactiae and genes encoding resistance to macrolides and lincosamides

Fig. 3

Sensitivity of multiplex real-time PCR assays. a LMC UEL 65 (cfb); (b) LMC UEL 65 [erm(A)]; (C) LMC UEL 66 [erm(B)]; (d) LMC UEL 15 [mef(A/E)]; (e) LMC UEL 15 [cfb and mef(A/E)]; (f) LMC UEL 66 [cfb and erm(B)]. Amplification plot of 10-fold serial dilution corresponding to 104–107 CFU; standard curve represented by linear regression line for threshold cycle (Ct) versus sample log concentration. Slope, regression coefficient and efficiency of the real-time PCR method are noted (a-f)

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