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Table 1 Panel of microorganisms used to evaluate the multiplex real-time PCR specificity and sensitivity

From: Development of a melting-curve based multiplex real-time PCR assay for simultaneous detection of Streptococcus agalactiae and genes encoding resistance to macrolides and lincosamides

Species Source Species Source
Streptococcus agalactiae ATCC 13813 Escherichia coli ATCC 25922
Streptococcus agalactiae LMC UEL 15 Escherichia coli ATCC 35218
Streptococcus agalactiae LMC UEL 65 Klebsiella pneumoniae ATCC 700603
Streptococcus agalactiae LMC UEL 66 Proteus mirabilis HU-UEL
Streptococcus agalactiae serotype Ia LMC UEL 43 Providencia stuartii HU-UEL
Streptococcus agalactiae serotype II LMC UEL 92 Salmonella sp. HU-UEL
Streptococcus agalactiae serotype III LMC UEL 59 Shigella dysenteriae ATCC 13313
Streptococcus agalactiae serotype V LMC UEL 73 Enterococcus faecalis ATCC 29212
Streptococcus agalactiae serotype IX LMC UEL 11 Enterococcus faecium ATCC 6569
Streptococcus dysgalactiae subsp. equisimilis group G. LACEN 6196 Lactobacillus acidophilus ATCC 4356
Streptococcus dysgalactiae subsp. equisimilis group C LACEN 53157 Lactobacillus rhamnosus LBBA-UEL
Streptococcus mitis ATCC 49456 Lactococcus lactis subsp. lactis LBBA-UEL 22
Streptococcus mutans ATCC 25175 Lactococcus lactis subsp. cremoris LBBA-UEL 22–1
Streptococcus pneumoniae ATCC 49619 Leuconostoc mesenteroides LBBA-UEL 704
Streptococcus pyogenes ATCC 19615 Candida albicans ATCC 26790
Streptococcus sanguis ATCC10557 Candida bracarensis LMC UEL1217
Staphylococcus aureus ATCC 25923 Candida dubliniensis LMC UEL 947C
Staphylococcus epidermidis ATCC 12228 Candida glabrata LMC UEL 51B
Staphylococcus haemolyticus ATCC 29668 Candida metapsilosis LMC UEL 2263
Staphylococcus saprophyticus HU-UEL Candida orthopsilosis LMC UEL 2259
Bacillus subtilis ATCC 23857 Candida parapsilosis ATCC 22019
Aeromonas sp. HU-UEL Candida tropicalis ATCC 28707
Pseudomonas aeruginosa ATCC 27853 Cryptococcus gattii ATCC 56990
Citrobacter freundii HU-UEL Cryptococcus neoformans ATCC 66031
  1. ATCC American Type Culture Collection, LMC Laboratório de Microbiologia Clínica, UEL Universidade Estadual de Londrina, LACEN Laboratório Central do Estado do Paraná, HU Hospital Universitário de Londrina, LBBA Laboratório de Bacteriologia Básica e Aplicada