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Table 2 Oligonucleotide primers of melting curve-based multiplex real-time PCR

From: Development of a melting-curve based multiplex real-time PCR assay for simultaneous detection of Streptococcus agalactiae and genes encoding resistance to macrolides and lincosamides

Targeta Nucleotide sequence (5′ to 3′) Amplicon size (bp) Reference
cfb b F: CACACATGCTGTTGGAGTTCAGTTGA 138 This study
R: ACGAAGTCGACAGCATCACACGAAA  
erm(A)/(TR) F: CCGGCAAGGAGAAGGTTATAATGA 190 Otaguiri et al. [5]
R: GCATTCACCCGTTGACTCATTTCC  
erm(B) F: GCTCTTGCACACTCAAGTCTCGAT 117 Otaguiri et al. [5]
R: ACATCTGTGGTATGGCGGGTAAGT  
mef(A/E) F: GCGATGGTCTTGTCTATGGCTTCA 225 Otaguiri et al. [5]
R: AGCTGTTCCAATGCTACGGAT  
RNaseP F: AGATTTGGACCTGCGAGCG 64 WHO [27]
R: GAGCGGCTGTCTCCACAAGT  
IGS1 F: GTCATTTCAGCTGGCGCCATCGATAC 260 Tavares et al. [32]
R: TTGCCGCATAACGCATCTTAGCCA   
  1. acfb gene encodes the CAMP factor; erm genes encode 23S rRNA methylases; mef gene encodes efflux pumps; RNaseP gene encodes human ribonuclease P; IGS1, intergenic spacer 1 of ribosomal RNA gene cluster of Cryptococcus gattii. bThe nucleotide sequences of Streptococcus agalactiae genes deposited in the GenBank/EMBL databases were used for specific primer design